First, may I tell you my husband is a retired Allergist and Immunologist and he observed my IBS experiences then elimination of them. For more than 40 years I had diarrhea and severe intestinal pain. The symptoms changed as the years passed. It went from occasional to frequent and from mild to severe. This leads me to believe it is dose and frequency related. The four years prior to my discovery that annatto was the problem for me, it was daily and severe. I would get an excruciating stomach ache with bloating after 20 minutes of ingestion of an annatto product. After another 20 minutes I had an emergency run to the WC and a long bout with painful diarrhea followed by many more trips to the WC. Then, I was enervated and just wanted to do nothing and sometimes for days after. I wasn’t lactose intolerant nor did I have Celiac Sprue (which the doctors considered). In 2005, after a colonoscopy, my GI diagnosed me with IBS. The symptoms continued until 2006, when I was able to pinpoint what caused my IBS symptoms.